The difference between moist heat sterilization and dry heat sterilization
The difference between moist heat sterilization and dry heat sterilization.
(A) Dry heat sterilization
1. Burning: a thorough sterilization method, but only dead bodies and other scrap materials or.
2. Ablation: Direct flame sterilization , microbiology laboratory for inoculation in the ring, such as sterilize test tube mouth.
3. Dry roast: oven sterilization. Generally heated to 160 ¡æ ~ 170 ¡æ after 2 hours. Applicable to high temperature is not bad, not evaporation items
Such as glassware, porcelain and so on.
(B) Moist heat sterilization
1. Boiling: 1 atmospheric pressure, the boiling temperature is 100 ¡æ, the general body of bacteria killed by boiling them for 5 min. Bacillus bacteria usually need to boil 1 to 2 hours, have been killed. Adding 2% sodium carbonate in water, can increase the boiling point up to 105 ¡æ, the promotion of bacterial spore kill, but will also prevent rusting metal containers.
2. Pasteurization (pasteurization): a lower liquid temperature to kill pathogens, without affecting the nutritional composition and the sterile smell of the disinfection method. Heating 61.1 ~ 62.8 ¡æ and a half hour, and then used in milk and wine and other disinfection. 3. Intermittent sterilization method (fractional sterilization): is repeated several times using the flow of steam, to kill all vegetative bacteria and spores in the sterilization method. Specific practice is to place the items to be sterilized Arnold steam sterilizer circulation, 100 ¡æ heating 15 to 30 minutes, kill bacteria which the body, then placing an item in box at 37 ¡æ overnight to spore development propagules into the next day and through the flow of steam heating, so three times, all the bacteria can kill all propagules and spores.
4. High-pressure steam sterilization: Sterilization is the best and most widely used method of sterilization. Is confined in a steamer - Autoclaves carried out. Heating steam can not spill, container temperature vapor pressure increases with increased bactericidal power has been greatly enhanced. Usually 1.05kg/cm2 pressure, temperature of 121.3 ¡æ, for 15 to 30 minutes, including bacterial spores can kill all microorganisms, including.
This method is suitable for high temperature and sterilization which are not afraid of wet items.
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